Using chemical shift perturbation to characterise ligand binding.

Using chemical shift perturbation to characterise ligand binding.

Chemical shift perturbation (CSP, chemical shift mapping or complexation-induced modifications in chemical shift, CIS) follows modifications within the chemical shifts of a protein when a ligand is added, and makes use of these to decide the placement of the binding web site, the affinity of the ligand, and/or presumably the construction of the advanced. A key consider figuring out the looks of spectra throughout a titration is the change price between free and certain, or extra particularly the off-rate koff. When koff is bigger than the chemical shift distinction between free and certain, which generally equates to an affinity Kd weaker than about 3μM, then change is quick on the chemical shift timescale.

Under these circumstances, the noticed shift is the population-weighted common of free and certain, which permits Kd to be decided from measurement of peak positions, offered the measurements are made appropriately. (1)H shifts are influenced to a big extent by through-space interactions, whereas (13) and (13)Cβ shifts are influenced extra by through-bond results. (15)N and (13)C’ shifts are influenced each by through-bond and by through-space (hydrogen bonding) interactions. For figuring out the placement of a certain ligand on the idea of shift change, probably the most acceptable methodology is subsequently normally to measure (15)N HSQC spectra, calculate the geometrical distance moved by the height, weighting (15)N shifts by an element of about 0.14 in contrast to (1)H shifts, and choose these residues for which the weighted shift change is bigger than the usual deviation of the shift for all residues.

Other strategies are mentioned, particularly the measurement of (13)CH3 indicators. Slow to intermediate change charges lead to line broadening, and make Kd values very troublesome to acquire. There isn’t any great way to distinguish modifications in chemical shift due to direct binding of the ligand from modifications in chemical shift due to allosteric change. Ligand binding at a number of websites can typically be characterised, by simultaneous becoming of many measured shift modifications, or extra just by including substoichiometric quantities of ligand. The chemical shift modifications can be utilized as restraints for docking ligand onto protein. By use of quantitative calculations of ligand-induced chemical shift modifications, it’s changing into potential to decide not simply the place but in addition the orientation of ligands.

Glutamate and neurotrophic elements in neuronal plasticity and illness.

Glutamate’s position as a neurotransmitter at synapses has been recognized for 40 years, however glutamate has since been proven to regulate neurogenesis, neurite outgrowth, synaptogenesis, and neuron survival within the creating and grownup mammalian nervous system. Cell-surface glutamate receptors are coupled to Ca(2+) inflow and launch from endoplasmic reticulum shops, which causes speedy (kinase- and protease-mediated) and delayed (transcription-dependent) responses that change the construction and performance of neurons. Neurotrophic elements and glutamate work together to regulate developmental and grownup neuroplasticity. For instance, glutamate stimulates the manufacturing of brain-derived neurotrophic issue (BDNF), which, in flip, modifies neuronal glutamate sensitivity, Ca(2+) homeostasis, and plasticity.

Neurotrophic elements could modify glutamate signaling straight, by altering the expression of glutamate receptor subunits and Ca(2+)-regulating proteins, and likewise not directly by inducing the manufacturing of antioxidant enzymes, energy-regulating proteins, and antiapoptotic Bcl-2 relations. Excessive activation of glutamate receptors, beneath situations of oxidative and metabolic stress, could contribute to neuronal dysfunction and degeneration in ailments starting from stroke and Alzheimer’s illness to psychiatric issues. By enhancing neurotrophic issue signaling, environmental elements comparable to train and dietary vitality restriction, and chemical compounds comparable to antidepressants could optimize glutamatergic signaling and shield in opposition to neurological issues.

Using chemical shift perturbation to characterise ligand binding.

Glial cells in (patho)physiology.

Neuroglial cells outline mind homeostasis and mount protection in opposition to pathological insults. Astroglia regulate neurogenesis and improvement of mind circuits. In the grownup mind, astrocytes enter into intimate dynamic relationship with neurons, particularly at synaptic websites the place they functionally kind the tripartite synapse. At these websites, astrocytes regulate ion and neurotransmitter homeostasis, metabolically help neurons and monitor synaptic exercise; one of many readouts of the latter manifests in astrocytic intracellular Ca(2+) indicators. This type of astrocytic excitability can lead to launch of chemical transmitters by way of Ca(2+) -dependent exocytosis.

Once within the extracellular area, gliotransmitters can modulate synaptic plasticity and trigger modifications in habits. Besides these physiological duties, astrocytes are elementary for development and final result of neurological ailments. In Alzheimer’s illness, for instance, astrocytes could contribute to the etiology of this dysfunction. Highly deadly glial-derived tumors use signaling trickery to coerce regular mind cells to help tumor invasiveness. This evaluation not solely sheds new gentle on the mind operation in well being and illness, but in addition factors to many unknowns.

Caspase 14 p10 antibody

70R-50903 100 ul
EUR 244
Description: Purified Polyclonal Caspase 14 p10 antibody

Anti-Caspase-1(P10)/CASP1 Antibody

PA1522 100ug/vial
EUR 334

Caspase 1 (p10, Cleaved-Ala317) Antibody

20-abx015550
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Cleaved Caspase-1 (Arg317) /P10 Antibody

AF4022 200ul
EUR 376
Description: Caspase 1 (p10,Cleaved-Arg317) Antibody detects endogenous levels of fragment of activated Caspase 1 resulting from cleavage adjacent to Arg317.

Caspase 1 p10 Blocking Peptide

20-abx062325
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Anti-Caspase-3 (P10)/CASP3 Antibody

PA1302 100ug/vial
EUR 294

Anti-Caspase-3 (P10)/CASP3 Antibody

PA1302-2 100ug/vial
EUR 334

Anti-Caspase-8(P10)/CASP8 Antibody

PA1524 100ug/vial
EUR 334

Caspase 14 (p10, Cleaved-Lys222) Antibody

20-abx015552
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Caspase 5 (p10, Cleaved-Ser331) Antibody

20-abx015560
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Caspase 5 p10 Blocking Peptide

20-abx062339
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Caspase 14 p10 Blocking Peptide

20-abx063778
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Cleaved Caspase-1 (Arg317) /P10 Blocking Peptide

AF4022-BP 1mg
EUR 195

Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

ES1020-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Cleaved-Caspase-5 p10 (S331) from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

ES1020-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Cleaved-Caspase-5 p10 (S331) from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

ABP50021-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Cleaved-Caspase-5 p10 S331) from Human. This Cleaved-Caspase-5 p10 S331) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Caspase-5 p10 at AA range: 290-370

Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

ABP50021-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Cleaved-Caspase-5 p10 S331) from Human. This Cleaved-Caspase-5 p10 S331) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Caspase-5 p10 at AA range: 290-370

Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

ABP50021-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Cleaved-Caspase-5 p10 S331) from Human. This Cleaved-Caspase-5 p10 S331) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Caspase-5 p10 at AA range: 290-370

Anti-Cleaved-Caspase-5 p10 (S331) antibody

STJ90029 200 µl
EUR 197
Description: Rabbit polyclonal to Cleaved-Caspase-5 p10 (S331).

Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

40509-100ul 100ul
EUR 252

Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

40509-50ul 50ul
EUR 187

Anti-Caspase-1(P20)/CASP1 Antibody

PA1440-1 100ug/vial
EUR 294

Anti-Caspase 8/CASP8 Antibody

A00042-1 100ug/vial
EUR 294

Anti-Caspase-2/CASP2 Antibody

A02384-1 100ug/vial
EUR 334

Anti-Caspase 4/CASP4 Antibody

A02941-1 100ug/vial
EUR 334

Anti-Caspase-8 Rabbit Monoclonal Antibody

M00042-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Caspase-8 Antibody. Validated in IF, WB and tested in Human.

Anti-Caspase-7 Rabbit Monoclonal Antibody

M01044-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Caspase-7 Antibody. Validated in IP, WB and tested in Mouse, Rat.

Anti-Caspase-10 Rabbit Monoclonal Antibody

M02190-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Caspase-10 Antibody. Validated in IP, WB and tested in Human.

Anti-Caspase-2 Rabbit Monoclonal Antibody

M02384-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Caspase-2 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Caspase-6(P18)/CASP6 Antibody

PA1441-1 100ug/vial
EUR 334

Anti-Caspase-3(P17)/CASP3 Antibody

PA1961-1 100ug/vial
EUR 334

Caspase-1 Antibody

24290-100ul 100ul
EUR 390

Caspase-1 Antibody

24291-100ul 100ul
EUR 390

caspase 1 Antibody

39310-100ul 100ul
EUR 390

Caspase-1 Antibody

48847-100ul 100ul
EUR 333

Caspase-1 Antibody

48847-50ul 50ul
EUR 239

Caspase 1 Antibody

AF5418 200ul
EUR 304
Description: Caspase 1 Antibody detects endogenous levels of total Caspase 1.

Caspase 1 Antibody

ABF5418 100 ug
EUR 438

Caspase 1 Antibody

6687-100
EUR 338

Caspase 1 Antibody

6687-30T
EUR 146

Caspase-1 Antibody

3019-100
EUR 403

Caspase-1 Antibody

3019-30T
EUR 146

Caspase 1 antibody

PAab10015 100 ug
EUR 386

Caspase 1 antibody

20R-1427 100 ug
EUR 651
Description: Rabbit polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-15367 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 1 antibody

Caspase-1 Antibody

3459-002mg 0.02 mg
EUR 171.82
Description: Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Caspase-1 Antibody

3459-01mg 0.1 mg
EUR 436.42
Description: Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Caspase-1 Antibody

3463-002mg 0.02 mg
EUR 171.82
Description: Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Caspase-1 Antibody

3463-01mg 0.1 mg
EUR 436.42
Description: Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Caspase 1 antibody

70R-11561 100 ug
EUR 527
Description: Rabbit polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-13979 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-14061 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-31086 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-31087 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-31116 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-49451 100 ul
EUR 244
Description: Purified Polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-49452 100 ul
EUR 244
Description: Purified Polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-51474 100 ul
EUR 244
Description: Purified Polyclonal Caspase 1 antibody

Caspase 1 antibody

70R-35920 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 1 antibody

3-D Life RGD Peptide

P10-3 3x 1 µmol
EUR 276

Anti-pro Caspase 9 Rabbit Monoclonal Antibody

M00080-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal pro Caspase 9 Antibody. Validated in WB and tested in Human.

Anti-pro Caspase 3 Rabbit Monoclonal Antibody

M00334-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal pro Caspase 3 Antibody. Validated in IP, IF, WB and tested in Human, Mouse.

Anti-Caspase-6 p18 Rabbit Monoclonal Antibody

M02631-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Caspase-6 p18 Antibody. Validated in IP, WB and tested in Human.

Caspase Inhibitor Set I

A9901-1 1 Set
EUR 154

Caspase-3/7 Inhibitor

2780-1
EUR 153

Caspase-3/7 Inhibitor I

A1925-1 1 mg
EUR 148
Description: Caspase-3/7 inbibitor I is a potent, reversible, isatin sulfonamide-based inhibitor of caspase-3 (KI(app) = 60 nM) and caspase-7 (KI(app) = 170 nM). Is a weaker inhibitor of caspase-9 (Ki(app) = 3.1 mM).

Caspase-1 Polyclonal Antibody

ES7677-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Caspase-1 from Human/Monkey. This antibody is tested and validated for WB, ELISA, WB, ELISA

Caspase-1 Polyclonal Antibody

ES7677-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Caspase-1 from Human/Monkey. This antibody is tested and validated for WB, ELISA, WB, ELISA

Caspase-1 Polyclonal Antibody

ES8461-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Caspase-1 from Human/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Caspase-1 Polyclonal Antibody

ES8461-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Caspase-1 from Human/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Caspase 1 (CASP1) Antibody

20-abx301352
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Caspase 1 (pS376) Antibody

abx148922-100ug 100 ug
EUR 439

Caspase-1 Polyclonal Antibody

ES1850-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Caspase-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Caspase-1 Polyclonal Antibody

ES1850-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Caspase-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Anti-Caspase-1 Antibody

A00048 100ug
EUR 432
Description: Rabbit Polyclonal Caspase-1 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Caspase 1 (CASP1) Antibody

20-abx109605
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Caspase 1 (CASP1) Antibody

20-abx100442
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 1 (CASP1) Antibody

20-abx100443
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 1 (CASP1) Antibody

20-abx100444
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 1 (CASP1) Antibody

20-abx100445
  • EUR 439.00
  • EUR 133.00
  • EUR 1247.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 1 (CASP1) Antibody

20-abx100446
  • EUR 467.00
  • EUR 133.00
  • EUR 1358.00
  • EUR 648.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 1 (CASP1) Antibody

abx037580-100ug 100 ug
EUR 391

Caspase 1 (CASP1) Antibody

abx038263-100ug 100 ug
EUR 391

Caspase 1 (CASP1) Antibody

20-abx007565
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 1 p20 Antibody

20-abx009515
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 1 (CASP1) Antibody

20-abx009516
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 1 (CASP1) Antibody

20-abx009517
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 1 (CASP1) Antibody

20-abx000918
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Caspase-1 Polyclonal Antibody

ABP50851-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Caspase-1 from Human, Mouse, Rat. This Caspase-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-1 around the non-phosphorylation site of S376

Caspase-1 Polyclonal Antibody

ABP50851-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Caspase-1 from Human, Mouse, Rat. This Caspase-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-1 around the non-phosphorylation site of S376

Caspase-1 Polyclonal Antibody

ABP50851-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Caspase-1 from Human, Mouse, Rat. This Caspase-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-1 around the non-phosphorylation site of S376

Caspase-1 Polyclonal Antibody

ABP56678-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Caspase-1 from Human, Monkey. This Caspase-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Caspase-1 at AA range: 270-350

Caspase-1 Polyclonal Antibody

ABP56678-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Caspase-1 from Human, Monkey. This Caspase-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Caspase-1 at AA range: 270-350

Caspase-1 Polyclonal Antibody

ABP56678-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Caspase-1 from Human, Monkey. This Caspase-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Caspase-1 at AA range: 270-350

A brand new program, TALOS-N, is launched for predicting protein spine torsion angles from NMR chemical shifts. The program depends much more extensively on the usage of educated synthetic neural networks than its predecessor, TALOS+. Validation on an impartial set of proteins indicates that spine torsion angles can be predicted for a bigger, ≥90 % fraction of the residues, with an error price smaller than ca 3.5 %, utilizing an acceptance criterion that’s practically two-fold tighter than that used beforehand, and a root imply sq. distinction between predicted and crystallographically noticed (ϕ, ψ) torsion angles of ca 12º. TALOS-N additionally reviews sidechain χ(1) rotameric states for about 50 % of the residues, and a consistency with reference constructions of 89 %.

Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites.

Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites.

Three units of conserved nucleotides in 23 rRNA are shielded from chemical probes by binding of tRNA to the ribosomal A, P, and E websites, respectively. They are situated nearly solely in area V, primarily in or adjoining to the loop recognized with the peptidyl transferase operate. Some of these websites are additionally protected by antibiotics equivalent to chloramphenicol, which might clarify how these medicine intervene with protein synthesis. Certain tRNA-dependent protections are abolished when the 3′-terminal A or CA or 2′,3′-linked acyl group is eliminated, offering direct proof for the interplay of the conserved CCA terminus of tRNA with 23S rRNA.

When the EF-Tu.GTP.aminoacyl-tRNA ternary advanced is sure to the ribosome, no tRNA-dependent A web site protections are detected in 23S rRNA till EF-Tu is launched. Thus, EF-Tu prevents interplay of the 3′ terminus of the incoming aminoacyl-tRNA with the peptidyl transferase area of the ribosome throughout anticodon choice, thereby allowing translational proofreading. Some PLTS cells appeared to have two axonal origins from the somata and dendrites. LA cells had been principally massive aspiny cells with Ca(2+)-dependent long-lasting afterhyperpolarizations and robust time-dependent hyperpolarizing rectification.

Plants have advanced subtle programs to manage with herbivore challenges. When vegetation understand herbivore-derived bodily and chemical cues, equivalent to elicitors in bugs’ oral secretions and compounds in oviposition fluids, vegetation dramatically reshape their transcriptomes, proteomes, and metabolomes. All these herbivory-induced modifications are mediated by elaborate signaling networks, which embrace receptors/sensors, Ca(2+) influxes, kinase cascades, reactive oxygen species, and phytohormone signaling pathways. Furthermore, herbivory induces protection responses not solely in the wounded areas but additionally in undamaged areas in the attacked leaves and in distal intact (systemic) leaves.

Here, we overview current progress in understanding plant notion of herbivory and oviposition, and the herbivory-induced early signaling occasions and their organic features. We think about the intraspecific phenotypic variety of plant responses to herbivory and focus on the underlying genetic variation. We additionally focus on new instruments and technical challenges in learning plant-herbivore interactions. Our outcomes point out that TRPA1 is an oxidant sensor in sensory neurons, initiating neuronal excitation and subsequent physiological responses in vitro and in vivo.

The impact of cyanide on the efflux of calcium from squid axons.

The common charge fixed for loss of (45)Ca from an unpoisoned squid axon was 1.Eight x 10(-3) min(-1), similar to an efflux of 0.2 p-mole/cm(2) sec.2. The Ca efflux from unpoisoned axons was diminished if exterior calcium was changed with magnesium, or exterior sodium with lithium, choline or dextrose. Replacing each sodium and calcium diminished the efflux to about 40%.3. Cyanide triggered little quick change in Ca efflux however after 1(half of)-2(half of) hr the efflux elevated to 5-15 instances its regular worth. The impact was quickly reversed when cyanide was eliminated.4.

The giant Ca efflux into cyanide was diminished by an element of three when exterior calcium was changed with magnesium and by an extra issue of about six when exterior sodium was changed with lithium.5. The Ca efflux from each poisoned and unpoisoned axons had a Q(10) of 2-3, was not affected by ouabain and was vastly diminished by injecting ethyleneglycol bis (aminoethylether)-N,N’-tetra-acetic acid (EGTA).6. After injecting (45)Ca alongside the axis, the efflux of calcium reached its most rather more quickly in a cyanide-treated axon than in an unpoisoned axon.7. Pre-treatment with cyanide vastly elevated the charge at which calcium was misplaced from axoplasm extruded into flattened dialysis luggage.

The same impact was noticed when cyanide was utilized after extrusion.8. Replacing exterior sodium glutamate with potassium glutamate vastly diminished the loss of (45)Ca from intact axons poisoned with cyanide however had little impact on the loss from extruded axoplasm.9. The charge fixed for loss of the Ca EGTA advanced was about Three x 10(-5) min(-1) for intact axons and 2 x 10(-2) min(-1) for extruded axoplasm.10. A doable clarification of the cyanide impact is that, after poisoning, calcium ions are launched from a retailer and can then change at a better charge with exterior sodium or calcium.11. The experiments counsel that half of the calcium efflux could also be coupled to sodium entry.12. Theoretical equations for ;diffusion and chemical response in a cylinder’ are described in the Appendix.

Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites.

TRPA1 is a serious oxidant sensor in murine airway sensory neurons.

Sensory neurons in the airways are finely tuned to answer reactive chemical substances threatening airway operate and integrity. Nasal trigeminal nerve endings are significantly delicate to oxidants shaped in polluted air and throughout oxidative stress in addition to to chlorine, which is steadily launched in industrial and home accidents. Oxidant activation of airway neurons induces respiratory melancholy, nasal obstruction, sneezing, cough, and ache. While usually protecting, chemosensory airway reflexes can provoke extreme issues in sufferers affected by inflammatory airway circumstances like rhinitis and bronchial asthma.

Caspase-9 Antibody

24050-100ul 100ul
EUR 390

Caspase-9 Antibody

48161-100ul 100ul
EUR 333

Caspase-9 Antibody

48161-50ul 50ul
EUR 239

Caspase-9 Antibody

48687-100ul 100ul
EUR 333

Caspase-9 Antibody

48687-50ul 50ul
EUR 239

Caspase-9 Antibody

49014-100ul 100ul
EUR 333

Caspase-9 Antibody

49014-50ul 50ul
EUR 239

Caspase 9 Antibody

AF6348 200ul
EUR 304
Description: Caspase 9 Antibody detects endogenous levels of total Caspase 9.

Caspase 9 Antibody

ABF6348 100 ug
EUR 438

Caspase-9 Antibody

6670-100
EUR 338

Caspase-9 Antibody

6670-30T
EUR 146

Caspase-9 Antibody

3016-100
EUR 338

Caspase-9 Antibody

3016-30T
EUR 146

Caspase-9 Antibody

3136-100
EUR 327

Caspase-9 Antibody

3136-30T
EUR 146

Caspase-9 Antibody

3409-100
EUR 316

Caspase-9 Antibody

3409-30T
EUR 146

Caspase 9 antibody

20R-2497 50 ug
EUR 281
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-1425 100 ug
EUR 673
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-1468 100 ug
EUR 673
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-1472 100 ug
EUR 781
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-1557 100 ug
EUR 673
Description: Rabbit polyclonal Caspase 9 antibody

Caspase-9 Antibody

2071-002mg 0.02 mg
EUR 171.82
Description: Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Caspase-9 Antibody

2071-01mg 0.1 mg
EUR 436.42
Description: Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Caspase-9 Antibody

2073-002mg 0.02 mg
EUR 171.82
Description: Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Caspase-9 Antibody

2073-01mg 0.1 mg
EUR 436.42
Description: Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Caspase 9 antibody

70R-30659 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-31082 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-31083 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-31084 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-33457 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-33459 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-33461 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-11636 100 ug
EUR 418
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-11806 100 ug
EUR 403
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-13511 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-14132 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-51476 100 ul
EUR 244
Description: Purified Polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-49474 100 ul
EUR 244
Description: Purified Polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-49475 100 ul
EUR 244
Description: Purified Polyclonal Caspase 9 antibody

Caspase-9

GT15045 100 ul
EUR 500

Caspase-9 Polyclonal Antibody

ES7743-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Caspase-9 Polyclonal Antibody

ES7743-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Caspase 9 Monoclonal Antibody

EM1077-100ul 100ul
EUR 279
Description: A Mouse Monoclonal antibody against Caspase 9 from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IP, IF

Caspase 9 Monoclonal Antibody

EM1077-50ul 50ul
EUR 207
Description: A Mouse Monoclonal antibody against Caspase 9 from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IP, IF

Caspase-9 Polyclonal Antibody

ES1860-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Caspase-9 Polyclonal Antibody

ES1858-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Caspase-9 Polyclonal Antibody

ES1858-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Caspase-9 Polyclonal Antibody

ES1859-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human/Monkey. This antibody is tested and validated for WB, ELISA, WB, ELISA

Caspase-9 Polyclonal Antibody

ES1859-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human/Monkey. This antibody is tested and validated for WB, ELISA, WB, ELISA

Caspase-9 Polyclonal Antibody

ES1860-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Caspase-9 from Human. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Polyclonal Caspase-9 Antibody

APG02416G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 . This antibody is tested and proven to work in the following applications:

Polyclonal Caspase-9 Antibody

APG02417G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 . This antibody is tested and proven to work in the following applications:

Polyclonal Caspase-9 Antibody

APG02418G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 . This antibody is tested and proven to work in the following applications:

Polyclonal Caspase-9 Antibody

APG02419G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 . This antibody is tested and proven to work in the following applications:

Monoclonal Caspase-9 Antibody

APG02420G 0.1ml
EUR 484
Description: A Monoclonal antibody against Human Caspase-9. The antibodies are raised in Mouse. This antibody is applicable in WB

Polyclonal Caspase-9 Antibody

APR00030G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 . This antibody is tested and proven to work in the following applications:

Polyclonal Caspase-9 Antibody

APR00097G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 . This antibody is tested and proven to work in the following applications:

Anti-Caspase-9 antibody

STJ11100939 100 µl
EUR 393
Description: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Caspase-9 Polyclonal Antibody

ABP50859-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Caspase-9 from Human, Mouse, Rat. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of T125

Caspase-9 Polyclonal Antibody

ABP50859-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Caspase-9 from Human, Mouse, Rat. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of T125

Caspase-9 Polyclonal Antibody

ABP50859-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Caspase-9 from Human, Mouse, Rat. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of T125

Caspase-9 Polyclonal Antibody

ABP50860-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Caspase-9 from Human, Monkey. This Caspase-9 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of S144

Caspase-9 Polyclonal Antibody

ABP50860-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Caspase-9 from Human, Monkey. This Caspase-9 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of S144

Caspase-9 Polyclonal Antibody

ABP50860-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Caspase-9 from Human, Monkey. This Caspase-9 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of S144

Caspase-9 Polyclonal Antibody

ABP50861-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Caspase-9 from Human. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of S196

Caspase-9 Polyclonal Antibody

ABP50861-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Caspase-9 from Human. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of S196

Caspase-9 Polyclonal Antibody

ABP50861-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Caspase-9 from Human. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of S196

Caspase-9 Polyclonal Antibody

ABP56744-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Caspase-9 from Human, Monkey. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of Y153

Caspase-9 Polyclonal Antibody

ABP56744-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Caspase-9 from Human, Monkey. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of Y153

Caspase-9 Polyclonal Antibody

ABP56744-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Caspase-9 from Human, Monkey. This Caspase-9 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of Y153

Caspase 9 (CASP9) Antibody

abx125415-50ul 50 ul
EUR 411

Caspase 9 (CASP9) Antibody

20-abx125612
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Caspase 9 (CASP9) Antibody

20-abx128531
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 9 (CASP9) Antibody

20-abx128921
  • EUR 398.00
  • EUR 133.00
  • EUR 1094.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 9 p35 Antibody

20-abx121860
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 9 (CASP9) Antibody

20-abx111427
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Caspase 9 (CASP9) Antibody

20-abx123445
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Caspase 9 Monoclonal Antibody

ABM40077-003ml 0.03ml
EUR 158
Description: A monoclonal antibody for detection of Caspase 9 from Human, Mouse, Rat. This Caspase 9 antibody is for WB, IHC-P, IF, IP. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

Caspase 9 Monoclonal Antibody

ABM40077-01ml 0.1ml
EUR 289
Description: A monoclonal antibody for detection of Caspase 9 from Human, Mouse, Rat. This Caspase 9 antibody is for WB, IHC-P, IF, IP. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

Caspase 9 Monoclonal Antibody

ABM40077-02ml 0.2ml
EUR 414
Description: A monoclonal antibody for detection of Caspase 9 from Human, Mouse, Rat. This Caspase 9 antibody is for WB, IHC-P, IF, IP. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

Caspase 9 (CASP9) Antibody

20-abx002046
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Caspase 9 (CASP9) Antibody

20-abx100471
  • EUR 300.00
  • EUR 133.00
  • EUR 759.00
  • EUR 398.00
  • EUR 258.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 9 (CASP9) Antibody

20-abx100472
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 9 (CASP9) Antibody

20-abx100473
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Caspase 9 (CASP9) Antibody

abx037417-100ug 100 ug
EUR 391

Caspase 9 (CASP9) Antibody

20-abx134447
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 9 p35 Antibody

20-abx141270
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 9 (CASP9) Antibody

20-abx136140
  • EUR 495.00
  • EUR 704.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 50 ul

Caspase 9 (CASP9) Antibody

20-abx007563
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 9 (CASP9) Antibody

20-abx009495
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 9 (CASP9) Antibody

20-abx009496
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Caspase 9 (pT125) Antibody

abx012082-100ug 100 ug
EUR 439

Caspase 9 (pS196) Antibody

abx031837-400ul 400 ul
EUR 523

Caspase 9 (pS196) Antibody

abx031837-80l 80 µl
EUR 286

Caspase 9 (CASP9) Antibody

abx033801-400ul 400 ul
EUR 523

Here, we confirmed that each hypochlorite, the oxidizing mediator of chlorine, and hydrogen peroxide, a reactive oxygen species, activated Ca(2+) inflow and membrane currents in an oxidant-sensitive subpopulation of chemosensory neurons. These responses have been absent in neurons from mice missing TRPA1, an ion channel of the transient receptor potential (TRP) gene household. TRPA1 channels have been strongly activated by hypochlorite and hydrogen peroxide in main sensory neurons and heterologous cells. In exams of respiratory operate, Trpa1(-/-) mice displayed profound deficiencies in hypochlorite- and hydrogen peroxide-induced respiratory melancholy in addition to decreased oxidant-induced ache conduct.

Evaluation of the Infinium Methylation 450K technology.

Evaluation of the Infinium Methylation 450K technology.
Studies of DNA methylomes maintain monumental promise for biomedicine however are hampered by the technological challenges of analyzing many samples cost-effectively. Recently, a serious extension of the earlier Infinium HumanMethylation27 BeadChip® (Illumina, Inc. CA, USA), known as Infinium HumanMethylation450 (Infinium Methylation 450Ok; Illumina, Inc. CA, USA) was developed. This upgraded expertise is a hybrid of two totally different chemical assays, the Infinium I and Infinium II assays, permitting (for 12 samples in parallel) evaluation of the methylation standing of greater than 480,000 cytosines distributed over the complete genome.
We used Infinium Methylation 450Ok to profile: first, the well-characterized HCT116 wild-type and double-knockout cell traces after which, 16 breast tissue samples (together with eight regular and eight main tumor samples). Absolute methylation values (β-values) had been extracted with the GenomeStudio™ software program after which subjected to detailed evaluation. In this text, we consider Infinium Methylation 450Ok on cell traces and tissue samples, highlighting some of its benefits but in addition some of its limitations. In explicit, we examine the methylation values of the Infinium I and Infinium II assays.
While this expertise appeared extremely sturdy as beforehand proven, we seen a divergence between the β-values retrieved from the sort I and kind II Infinium assays. Specifically, the β-values obtained from Infinium II probes had been much less correct and reproducible than these obtained from Infinium I probes. This means that information from the sort I and kind II assays must be thought of individually in any downstream bioinformatic evaluation. To have the ability to cope with the Infinium I and Infinium II information collectively, we developed and examined a brand new correction method, which we known as ‘peak-based correction’. The concept was to rescale the Infinium II information on the foundation of the Infinium I information. While this system must be seen as an approximation methodology, it considerably improves the high quality of Infinium II information.
Infinium 450Ok is a strong method in phrases of reagent prices, time of labor, pattern throughput and protection. It holds nice promise for the higher understanding of the epigenetic part in well being and illness. Yet, on account of the nature of its design comprising two totally different chemical assays, evaluation of the complete set of information will not be as straightforward as initially anticipated. Correction methods, reminiscent of the peak-based method proposed right here, are a step in the direction of enough output information evaluation.
Astrocytes set up speedy cell-to-cell communication via the launch of chemical transmitters. The underlying mechanisms and purposeful significance of this launch are, nonetheless, not properly understood. Here we determine an astrocytic vesicular compartment that’s competent for glutamate exocytosis. Using postembedding immunogold labeling of the rat hippocampus, we present that vesicular glutamate transporters (VGLUT1/2) and the vesicular SNARE protein, cellubrevin, are each expressed in small vesicular organelles that resemble synaptic vesicles of glutamatergic terminals.
Evaluation of the Infinium Methylation 450K technology.

Physiological, morphological, and histochemical characterization of three courses of interneurons in rat neostriatum.

Interneurons in lateral half of neostriatum had been studied in remoted slices from juvenile rats (16-20 d postnatal) by whole-cell, current-clamp recording at 33-34 levels C, adopted by intracellular staining with biocytin and double immunocytochemical or histochemical staining for parvalbumin, ChAT, and NADPH diaphorase. Medium-sized spiny neurons (MS cells) had distal dendrites with many spines and had been doubtless projection cells, whereas interneurons had dendrites with fewer spines. The neostriatal interneurons might be additional divided into three courses by physiological, chemical, and morphological standards.

The top notch of interneurons (fast-spiking cells, FS cells) fired very short-duration motion potentials with short-duration afterhyperpolarizations at fixed spike frequency throughout depolarizing present pulses. FS cells had extra destructive resting potentials and decrease enter resistances than the different two courses. At depolarized potentials, FS cells fired repetitive spikes in response to synaptic excitation. FS cells had been immunoreactive for parvalbumin. As all parvalbumin-immunoreactive cells in the neostriatum had been additionally immunoreactive for GABA, FS cells had been thought of to be GABAergic.

FS cells had been additional divided into two morphological varieties: FS cells with native dendritic fields and FS cells with prolonged dendritic fields. The axons of each varieties of FS cells had their densest collateralization inside or close to their dendritic fields. The different two courses of interneuron, PLTS cells (persistent and low-threshold spike cells) and LA cells (long-lasting afterhyperpolarization cells), had been distinguished from FS cells by longer-duration motion potentials and bigger enter resistances, had much less destructive resting potentials, and had longer-lasting afterhyperpolarizations.

CASC4 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV708321 1.0 ug DNA
EUR 316

CASC4 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV708325 1.0 ug DNA
EUR 316

CASC4 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV708326 1.0 ug DNA
EUR 316

Casc4 ORF Vector (Mouse) (pORF)

ORF040409 1.0 ug DNA
EUR 506

Casc4 ORF Vector (Mouse) (pORF)

ORF040410 1.0 ug DNA
EUR 506

Casc4 ORF Vector (Mouse) (pORF)

ORF040411 1.0 ug DNA
EUR 506

Casc4 ORF Vector (Mouse) (pORF)

ORF040412 1.0 ug DNA
EUR 506

Casc4 ORF Vector (Mouse) (pORF)

ORF040413 1.0 ug DNA
EUR 506

CASC4 ORF Vector (Human) (pORF)

ORF001883 1.0 ug DNA
EUR 95

CASC4 ORF Vector (Human) (pORF)

ORF001884 1.0 ug DNA
EUR 95

CASC4 cloning plasmid

CSB-CL764724HU1-10ug 10ug
EUR 233
Description: A cloning plasmid for the CASC4 gene.

CASC4 cloning plasmid

CSB-CL764724HU2-10ug 10ug
EUR 233
Description: A cloning plasmid for the CASC4 gene.

Mouse CASC4 shRNA Plasmid

20-abx983339
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human CASC4 shRNA Plasmid

20-abx964315
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

CASC4 Recombinant Protein (Mouse)

RP121223 100 ug Ask for price

CASC4 Recombinant Protein (Mouse)

RP121226 100 ug Ask for price

CASC4 Recombinant Protein (Mouse)

RP121229 100 ug Ask for price

CASC4 Recombinant Protein (Mouse)

RP121232 100 ug Ask for price

CASC4 Recombinant Protein (Mouse)

RP121235 100 ug Ask for price

CASC4 Recombinant Protein (Human)

RP005647 100 ug Ask for price

CASC4 Recombinant Protein (Human)

RP005650 100 ug Ask for price

CASC4 Protein Vector (Human) (pPB-C-His)

PV007529 500 ng
EUR 329

CASC4 Protein Vector (Human) (pPB-N-His)

PV007530 500 ng
EUR 329

CASC4 Protein Vector (Human) (pPM-C-HA)

PV007531 500 ng
EUR 329

CASC4 Protein Vector (Human) (pPM-C-His)

PV007532 500 ng
EUR 329

CASC4 Protein Vector (Human) (pPB-C-His)

PV007533 500 ng
EUR 329